Cell migration is a directional cell movement to specific location in response to external stimulation. In this paper, an active disturbance rejection cell migration control method is presented. This method is enabled by an automated single cell manipulation system that consists of optically controlled chemoattractant-loaded microsources. The effectiveness of the control strategy is verified by both simulation and experiment of migrating leukemia cancer cells. The results show that the cell can be induced to migrate to a desired position with a relatively active motility status.