This paper wasfocused on the ride-comfort. There are different effects in differentoperating conditions on the high-speed train. Based on the practical testing of the high-speed train, the changes of vibration on the high-speed train were researched in this paperin the conditions of operating on the railway or in the tunnel. With the frequency-domain analysis and time-domain analysis, the distribution of the vibration main frequency was given in the paper. According to UIC513R standard 《Guidelines for evaluating passenger comfort in relation to vibration in railway vehicles》, the ride-comfort value of different operating conditions was calculated and the ride-comfort grade wasevaluated.