Underwater speech communication plays an important role in many marine missions, such as oceanographic investigation, environmental monitoring, underwater rescuing and sightseeing. However, the difficulties of underwater acoustic channels such as multipath, time varying and Doppler shifting pose significant challenges to the design of high performance underwater acoustic speech communication. In this paper, time reversal time-frequency differential OFDM is proposed for underwater acoustic speech communication, which enables time-frequency domain focusing of multipath by multi-channel time reversal, as well as suppressing of the residual multipath with time-frequency differential OFDM. Finally, the quality of the sea trial speech communication is quantitatively evaluated via the PESQ (Perceptual evaluation of speech quality) method to demonstrate the effectiveness of the proposed method.