The Cornea is the most important layer in human eye which consists of 12 sub layers in it, Each one is very unique to its own identity and forming some diseases in it cause vigorous vision problems, This paper deals with the corneal diseases using NI VISION Assistant in LabVIEW. NI Vision for LabVIEW is a part of the NI VDM is a library of LabVIEW used to develop machine vision and scientific imaging applications. The diseases and disorders affecting the cornea are many but only few of them are discussed in this paper. They are: Corneal Infections, Dry Eye, Keratoconus, and Allergies.