We fabricated a totally flexible glucose biofuel cell containing conductive porous polypyrrole (PPy) electrodes modified with enzymes. The porous PPy films were galvanostatically synthesized and glued on a polyimide flexible sheet to form porous conductive electrodes. The surface of anode was modified with glucose oxidase and a ferrocene mediator, and the surface of cathode was deposited with bilirubin oxidase. The generated power in the biofuel cell with PPy electrodes was 5 times higher than that with carbon electrodes.