Plasma is the 4th state of matter, which can be generated by an electric discharge with a high voltage bias. Recently, the non-thermal plasmas (NTPs) generated in an atmospheric pressure have attracted many interests in bio-medical applications because the activated gases by plasmas as well as the charged particles have lots of chemical elements to stimulate various kinds of chemical reactions in living cells. Even though the electron temperature reaches up to tens of thousands degree to generate lots of chemical reactions in the air, the gas temperature of NTPs is still at room temperature, and thus no thermal damage is added on the cells. In this study, we report the application of NTPs in the field of dermatology, especially for skin renewal. Since LTPs have strong anti-pathogenic activity with safeness, they were applied for the sterilization of skin surface for healing of various kinds of skin diseases. The effect of each reaction species from the NTPs on the proliferation activity of human skin cell was investigated after then. It was found out that the effects of NTPs on the skin renewal are related to the proliferation of keratinocytes in the skin tissues. The repeated treatment of NTPs on skin stimulates skin renewal without damages. Therefore, this study elucidated a new possibility of developing new types of skin-regeneration stimulating devices. For a successful application of this technology to skin care market, bioinformatics technology is necessary to optimize many operation parameters such as the mixture of gases, operation time, bias voltage for plasma generation, device structures, and so on. In addition, the real-time diagnostics of the plasma and the surface status and data communication are another important issue.