This paper discusses the feasibility of a real-time active matching circuit (MC) for wireless power transfer applications, especially for biomedical systems. One prototype of low-cost real-time automatic MC, utilizing a variable circuit topology, including discrete passives and p-i-n diodes, has been implemented and the principle has been verified by measurements. One genetic algorithm was introduced to optimize the design over a wide range of impedances to match. As a result of preliminary operation verification tests, the proposed real-time MC system results in improving the transfer coefficient in the range of 10–16-cm coil separation distance a maximum of 3.2 dB automatically in about 64 ms. Similar performance improvement results were observed in additional tests under misaligned conditions, as well as for nonsymmetrical Tx–Rx coil configurations further verifying the potential applicability of the proposed system to practical biomedical devices.