The multilateral analysis of mouse electroencephalography (EEG) is essential for various fields such as neurology, preclinical research, and neural engineering. The systematic and quantitative analysis of visually evoked potential (VEP) elicited by the repeated flash stimuli provides a foundation for related applications. We confirm the experimental validation of the developed mouse EEG sensor through VEP analysis by successive two flash stimuli. The EEG signal by the first stimulus shows higher amplitude and more precise subdivision in temporal segmentation than second stimulation case. We have confirmed a high utilization of developed sensor for in vivo experiments, and we expect the results with the developed mouse EEG sensor could help enhancing the performance of related applications such as clinical system and brain-computer interface.