For the cylinder of the axial piston pump suffers complicated forces including hydraulic pressure force, mechanical forces and mutual coupling, which causes the cylinder fatigue damage with all forces whose fluctuation is caused by fluid striking on the cylinder of the pump cyclically, it is necessary to develop the fatigue analysis research. And the sensitive fatigue areas of the cylinder in the axial piston pump is presented through the nephogram of the fatigue life, the fatigue safety factor over the whole model and the curve of fatigue sensitivity, which can verify the theory of micro crack propagation and the transient dynamics analysis of the axial piston pump cylinder is simulated. All the results are produced by software, Solidworks is used to build the three-dimension assembling model containing seven pistons and ANSYS Workbench is used to manage the model through setting the parameters of different materials and constrains of the cylinder. It's promising to provide a theoretical basis when optimizing the design of the axial piston pump and to offer new ideas and methods for the study of reliability and fatigue durability on the axial piston pump.