This paper aims to research and determine GEO satellite clock bias during maneuvering. By analyzing of GEO satellite clock bias data, quadratic polynomials, cubic spline and Lagrange are chose as interpolation methods. The result of the test in this paper shows that in most cases, cubic spline interpolation is the best one of the three interpolation methods. And the accuracy of cubic spline interpolation is at the level of 0.08ns∼0.38ns which can meet the actual demand; besides the stability of cubic spline interpolation is obviously better than that of quadratic polynomials and Lagrange interpolations.