This paper reports micromachined piezoelectric-on-silicon bulk acoustic wave resonators operating at a nominal frequency of approximately 3.15 MHz in fluidic media. Electrical measurements of the open-loop response of the resonators when one of the resonator surfaces is submerged in water indicate high quality factors in the range of 110–190. These values of quality factor are at least an order of magnitude higher than the flexural mode counterparts. The resonators are further exposed to Glycerol-Water mixtures of varying viscosity-density resulting in characteristic negative resonant frequency shifts. Experimental values are compared with a simplified liquid loading model and an agreement of up to 13% for highest and within 3–4% for lowest glycerol concentrations is established. These devices due to the relative ease of operation in liquid environments, scalability, high quality-factors and high mass-sensitivity have the potential for integration with microfluidics and electronics in order to realize an integrated platform for biochemical sensing and analysis.