Implementation of Fuzzy Logic in sugar industry will provide a new method to overcome various problems and increase the overall efficiency of sugar industries. The fuzzification, rule base and defuzzification are the key elements of a fuzzy system. Apart from fuzzification and defining rule there are numerous ways of defuzzification. This paper deals with the analysis and a comparative study of different deffuzification methods used for a three input fuzzy controller. The fuzzy controller developed and simulated for different deffuzification method by using Fuzzy Tool Box provided by MATLAB version 7.7 (R2008b).