The second generation of Kinect sensor is a revolutionary multiple cameras including RGB camera, infrared camera to differentiate depth, infrared camera and audio array. In this paper, we first introduce the differences between the Kinect and Kinect2.0 sensor. Then a system of Kinect2.0 based human interaction system for mobile robot is presented. The function of skeleton tracking of Kinect2.0 is used to identify a specific person in the depth image. Finally, people's heart rate detection method is proposed using RGB camera of Kinect2.0. Preliminary experiments verify that the designed measurement system is effective.