This research aims to examine the effect of knowledge management on the excellence of the Research Center. The study was applied to the case of Scientific Research Centers in Algeria; such as; (CDER, CERISTI, CDTIA, CSC, CRAPC, CRSTIDLA, CREAD, CRASC, CRSTIRA and CRBt). The data of the study were collected through interviews and questionnaire during 2011–2013, and they were analyzed using SPSS 18.0 to determine the interaction between the various factors. The findings broadly support the hypotheses and suggest a number of insights about future studies.