The characteristic of microscopic vascular data is that it contains many tiny vessels with branching and complex structure. The quantification of these vascular networks is crucial in diagnose of vascular abnormalities, surgical planning, and monitoring disease progress or remission. Therefore, We have developed a computerized system for automatically measuring the vascular data of a microscopic image. The major component of this system is an image processing-based method for quantitative analysis and a fast and accurate vessels extraction method.