This paper presents an integrated offline testing of Single-Fault detection technique for the micro-fluidic based biochips and also diagnosis of single defects in order to achieve higher throughput and less time complexity of the execution process. In the field operation, this is to be used to increase chip dependability and reusability. In this paper, a pipelined technique is presented to traverse all the edges in much more less time in comparison to some previous approaches. It also ensures no collision of the droplets throughout the procedure.