In this paper, we propose three novel modulation techniques, i.e., concentration-based, molecular-type-based, and molecular-ratio-based, using isomers as messenger molecules for nano communication networks via diffusion. To evaluate achievable rate performance, we compare the proposed techniques with conventional insulin-based concepts under practical scenarios. Analytical and numerical results confirm that the proposed modulation techniques using isomers achieve higher data transmission rate performance than the insulin based concepts. We also investigate the tradeoff between messenger sizes and modulation orders and provide guidelines for selecting from among several possible candidates.