The effects of LED light at 635 nm in fibroblast and osteoblast were investigate in fundamental changes as preliminary experiment. In this study, primary gum fibroblast cells and osteoblast cell line were cultured. After LED light irradiation, we found that fibroblast growth rate and cell proliferation are increased after irradiated by 630nm LED light. Optimum energy for wound healing is 0.34 J/cm2 at 630 nm. Optimum energy for proliferation is 0.67 J/cm2 at 630 nm. However, osteoblasts do not significantly and effectively response to LED light at 630 nm.