Intense nanosecond electric pulses act on cells by charging their membranes to voltages which may cause membrane permeabilization by creating nanopores1. Previous studies were mostly focused on the effects of monophasic pulses, which have been shown to permeabilize the cell membrane mainly on the anode side of the cells2. Biphasic pulses, on the other hand, are expected to affect both sides of the cell thereby potentially increasing the efficiency of membrane permeabilization. However, depending on the pulse duration, it is also possible that the biological effect created by one part of the biphasic pulse may be reversed by the part of the biphasic pulse with opposite polarity, reducing the efficiency compared to that of monophasic pulses. In order to explore which effect dominates for bipolar pulses, more biological studies need to be conducted.