Microfluidic Impact Printing (MI-Printing), which combines the desired features from the non-contact cartridge design of the inkjet printing and the dot-matrix impact printing, has been established for emerging biomedical applications. Using this droplet micropatterning platform, we can achieve the distinct features of submicroliter reagent manipulation with minimal dead volume enabled by the interchangeable/disposable microfluidic cartridge design, self-alignment printing, complex medium printing (cell and colloidal solutions), no cross-contamination, high throughput, easy to microfabrication, assembly and configure, all highly desirable towards biological applications.