This paper presents a method for extracting regions of non-rigid moving objects from a video. The proposed method utilizes interactive image cutout and local affine transformation. In the proposed method, once a user draws seed-lines on the object and background regions of the first frame of a video, the seed-lines are automatically tracked to next frames based on local affine transformation, and the object regions in each frame are automatically extracted by image cutout using the tracked seed-lines. Experimental results have shown that the proposed method can correctly extract the regions of nonrigid objects from a video.