Fats are immensely important for the survival of the human body. These are stored in the body in the form of fatty acids and reused whenever the body is unable to consume essential fats(at the time of exercise, fasting etc). In the human heart cell, the transport of certain fatty acids into the mitochondria is required to produce energy. This is done via a certain enzyme called Carnitine Translocase. This paper emphasizes on the simulation of the presence of Carnitine Translocase. Metabolic flux balance analysis was done to detect the presence of this enzyme, thus by preventing the accumulation of excess Fatty Acyl Carnitine that is known to give rise to various Cardiovascular diseases such as hypoglycemia, arrhythmia etc.