This paper presents a novel multi-slicing of Caenorhabditis elegans (C. elegans) tissue using micro-nanocutting probe based on nanomanipulation. We have developed the nanorobotic manipulation system inside an Environmental-Scanning Electron Microscope (E-SEM). This microscope provides a real-time high resolution image under water-contained condition. We have been applied this system for nanobiomanipulation, nano-bioevaluation, and single cell-analysis/manipulation using various types of nanotools. To analyze a piece of biological tissue precisely, it is needed to slice in micro-nanometer sizes. In this paper, we propose the single or multiple micro-nanocutting probe to slice the tissue of C. elegans, which is one of the important model organism. The proposed technique will be useful to obtain the high-precision analysis of model organism and treatment of C. elegans organs partially for understanding and simulating biological system.