Based on interference sources, an anti-interference measurement method for the zero sequence parameters of transmission lines is studied. The principle, measurement method and calculation formula of this method are explained in detail. The hardware structure of the measurement system and digital simulation results are given. Finally, an example of using the method to measure the zero sequence parameters of two 500 kV transmission lines with mutual inductance in a power grid of East China is given. The measurement results are compared with the increment measurement results. Field measurement results have proven that this method can meet the needs of accurately measuring the zero sequence impedances of transmission lines.