Through inoculating Amorpha fruiticosa with arbuscular mycorrhizal (AM) fungi and without, the dynamic characteristics of plant endogenous hormone levels, soluble sugar contents in roots and in leaves respectively were systemic studied. The results showed that AM fungi could obviously influenced soluble sugar contents and endogenous hormone levels in host plants with colonization percentage changing. Two kinds of AM fungi, Glomus mosseae and Glomus intraradices, were chose as tested strains to infect A. fruiticosa roots for forming the mycorrhizal complex using pot-experiment methods, and G. mosseae had a better colonization effect than G. intraradices on A. fruiticosa roots. After 70 days growth of A. fruiticosa seedings in the greenhouse, the colonization percentage of G. mosseae on hosts reached 88.5% that of the highest. The soluble sugar contents in roots and leaves of seedlings inoculated with AM fungi were significantly higher than control treatments, and had positive correlation with colonization percentage. AM fungi dramatically increased the contents of auxin (IAA), gibberellin (GA) and zeatin (ZR) in roots, but had litter impact on abscisic acid (ABA).