Automated analysis of retinal images can assist in the diagnosis and management of blinding retinal diseases, such as diabetic retinopathy, age-related macular degeneration (AMD), and glaucoma. For evaluating and imaging patients with retinal diseases, clinical photographers usually capture color images of the retina using a specialized fundus camera. Subsequently, a fluorescein dye is injected into a vein in the subject's arm, and as the dye propagates through the retinal blood vessels, and series of (over a 5–10 min period) pictures of the retina are taken. Retinal fluorescein images at two different stages of Angiogram and a color fundus image of the same patient are used as input images for lesion diagnosis. From the two fluorescein images, vessel extraction is done, they are aligned and fused to identify the region of abnormality and lesion growth. From the color image, the 3D plot is simulated to identify the area of abnormality of the eye and is used as reference to the fused fluorescein image. In this paper we present a novel technique for diagnosis of lesions through Fluorescein Angiographic Images using Virtual Instrumentation(VI).