With the method of experimental analysis, 7Be Fallout and its distribution in soil profile in later spring was analyzed in Nanjing region. The results showed that: the specific activity of 7Be on later spring rain in Nanjing was 0.85–2.62Bq/L; the concentration of 7Be in rainwater appeared decreased gradually; the total atmospheric wet deposition of 7Be was 128.66 Bq/m2, and the contribution rate of 7Be in atmospheric dry deposition was smaller. The reference inventories of 7Be in the four dates were: 249.17Bq/m2, 234.77Bq/m2, 267.12Bq/m2 and 272.14Bq/m2, and the distribution trend of the reference inventories of 7Be in soil profile was decreasing exponentially with depth, the total depth of 20mm.