Inappropriate thrombus formation in blood vessels causes cardiovascular disease (myocardial infarction, stroke, pulmonary embolism and so on) which is the most common cause of mortality at present. Fxa is an important factor in blood coagulation cascade. It is an effective and safe method to treat blood coagulation diseases by inhibiting FXa to control blood coagulation. In this paper, a new anticoagulant activity FXa inhibitor was synthesized using polypeptide synthesis method in liquidoid from basic materials. Compared with other methods, the new synthesis method applies the fragments condensation can save cost and time, and yield of all reaction was enhanced.