Road freight industry undertaking cold chain logistics, shows enterprises an opportunity for enabling a rapid promotion to modern logistics industries, expanding the scope of commercial services, and benefiting their developments. For the special requirements of cold chain logistics, road freight transport companies must know and recognize the risks (existing or potential one) of cold chain logistics operations, and consequently take effective risk management techniques to solve their derived problems in advance. Based on the constructed criteria for evaluating the operational risks of undertaking cold chain logistics services, this report adopts the “fuzzy comprehensive decision-making” to carry out quantifiable evaluations of the operational risks in the road freight industry undertake cold chain logistics services.