According to the calculating methods given by Xie gaodi et al, this paper calculates the loss of the ecosystem services value, which is caused by the as-built highway of Shandong province, and the ecosystem services value of the land which will occupied by the proposed highway is estimated. The results indicate that the total loss of ecological services value caused by the construction of highway network in Shandong province is about 2526196900 yuan every year, the loss of the ecosystem services value caused by the as-built highways is about 1686249600 yuan every year, the loss caused by the proposed project is expected to 839947300 yuan every year. Among the loss of ecosystem services value caused by the highway network construction, the contribution of agro-ecosystem and forest ecosystems is the largest, accounting for 77.9% and 18.5% respectively. The contribution of waste treatment is the highest, accounting for 20.5%, followed by the formation and conservation value of soil, accounting for 20.2%.