The application of the Internet of things (IOT) in industry and mining enterprises is analyzed in this paper. A new type of IOT application layer reader with functions of RFID and data transfer by wireless sensor is designed, according to the characteristics of the Internet things of industry and mining enterprises. This reader can perceive information from people, machine and environment of industry and mining enterprises. Hardware structures, system composition and the main characteristics of the reader is described and analyzed, which lays the foundation for the application of IOT in industry and mining enterprises and obtains good results.