A special report from the American Heart Association (AHA), American College of Cardiology, and Society of Nuclear Medicine defined standards for plane selection and displays orientation for serial myocardial slices generated by cardiac 2-dimensional (2D) or tomographic imaging witch aimed to give a standardized myocardial nomenclature for CT images. This standardization gives also an efficient evaluation of the cardiac function. In fact, cardiac function allows doctors to have an idea about the extent and the severity of cardiac anomalies, their scalability and the treatment efficiency. Estimation of myocardial function still up to now subjective and depends on doctors opinions. In this work, we propose a quantitative analysis of the myocardium function namely defining the thickness quantification of the myocardium in two specific states of the cardiac cycle (end systole and end diastole) based on the AHA standardization.