The transmission and control system is the core of the latest-twisting machine. For overcoming the shortcoming of the single-chip control system, which is used in the latest-twisting machine, a multi-motor transmission and control system of Latest-twisting machine based on PCI bus is developed. The transmission system is a multi-motor transmission system of four motors. An appropriate mathematic model has been developed based on multi-twisting theory. The running of spindles, the winding of bobbins and the reciprocate of guiding-yarns are controlled in phase by the control system, which is based on the integration of the AC servo technology, the frequency conversion technology, the PCI local bus technology and the twisting theory. The Visual Basic program language is used to realize modularized design in the software system. The dynamic-link library of the PCI board is called by the main program. The experiment results shows that the design of the transmission and control system can achieve the process objectives of twisting and winding of the latest twisting machine.