Wheat is one of the most important crops in the world. So the safety of wheat product is essential to people's health. With imitating experiment of arsenic polluted soil in pots, effects of nitrogen and phosphorus fertilizers on yield and quality characters of wheat were studied, and arsenic contents of wheat root, stem, grain and soil were determined detailedly. The effective measures by adding nitrogen and phosphorus fertilizers against toxicity of arsenic were emphatically discussed in the paper. The result showed that nitrogen and phosphorus fertilizers improved wheat yield and promoted wheat protein contents to some extent. Arsenic content of grains in mature period was reduced obviously, but the arsenic content of soil, root and stem changed differently, up or down. On the other hand, protein content of wheat grain increased, but contents of protein components, including albumin, globulin, gliadin and glutenin, hardly varied. That is to say, nitrogen and phosphorus fertilizers had no contribution to contents of protein components in wheat grain.