The signal acquisition and processing of homogenizing pressure in high pressure homogenizer are studied. For the measuring requirements of dynamic parameters in high pressure homogenizer, pressure transmitter, the signal collecting circuits about intensifier pump's motion were selected. The signal acquisition system can perform the functions such as signal collecting, recording and data recalling and so on. Moreover, the wavelet decomposition using 'sym4' at level 10 was used for de-noising of homogenizing pressure signal. The decomposed signal is selected through Birge-Massart threshold strategy and reconstructed. After collected and processed, the homogenizing pressure signal can be the foundational data for analyzing the homogenizing process and improving the crucial structure of high pressure homogenizer.