Intermittent testing method used to study the operating characteristic of the two-phase anaerobic process dealing with the low concentration of wheat straw-containing wastewater. The wheat straw dosage within the acidogenic reactor and the change of pH, ammonia nitrogen, COD and VFA in the two-phase anaerobic reactor were investigated. The result indicated that two-phase anaerobic treatment was still in the range of its appropriate pH. In the acidogenic reactor, wheat straw dosage accounted for about 36% of the total wheat straw dosage, which could make the effluent COD concentration of acidogenic reactor remained a high level. Adding wheat straw could increase the effluent VFA of the acidogenic reactor, and the acidification rate of the acidogenic reactor could reach as high as 75.85% to 100.23%.The COD removal efficiency of wheat straw-containing wastewater ranged from 54.61% to 74.13%, the biogas production rate ranged from 0.21 m3/kgCODr to 0.35 m3/kgCODr in the methanogenic reactor.