Multiplexed patterns of hydrogels and phospholipids with fluorescent dyes are accomplished by dip-pen nanolithography?? (DPN??). For example, four different dyes-labeled hydrogel dot arrays are DPN?? patterned within 50 ?? 50 ??m2 area, and two different dyes-doped phospholipids dots and letters with less than 1 ??m line-width are also DPN?? patterned. We demonstrate that multi-ink patterns with precise alignment are able to be printed by DPN?? within a micron-scale. Moreover, this multi-ink DPN?? patterning methodology can be extended to delicate bio-materials printing in a subcellular scale with accurate positioning control.