How to improve the bioactivity of titanium is always the key factor in the application of titanium artificial implants. Among kinds of means to reinforce its bioactivity, deposition of bioactive layers on titanium substrates and anodic oxidation are two common and powerful means. In this study, we combined the two methods to construct a more bioactive titanium surface. Plasma spraying processed for macro pores formation on the titanium substrates and followed by anodic oxidation to prepare a layer of porous sub-micro titanium oxidation. The bioactivity of the samples was evaluated in vitro by soaking in simulated body fluid (SBF) solution. The results revealed that porous titanium coatings with anodic oxidation showed a sub-micro pore structure and showed better bioactivity.