Leaf blight is a common Camellia oleifera disease caused by Pestalotiopsis microspora. The damaged young leaves show blight symptom, resulting in yield reduction. This study was to isolate antagonistic bacteria from Camellia oleifera tissue for control of the leaf blight. Antagonistic bacteria was screened from 175 endophytic bacterias by confront culture method. The results of the study showed that the antagonistic effect of YB13 strain was the best with 84.7% inhibitory rate. According to the results of the researching on morphology, cultural characteristics, physiological and biochemical propertices, 16S gene sequence and molecular phylogenetic tree, YB13 was identified as Bacillus subtilis.