After the analysis of movement of subway under moving block system, the problem of operation adjustment is studied. The model of the problem is constructed. The objective are decreasing the total delay time and increasing the absorption to passengers of the successive trains. The technology of agent and multi-agent is adopted in the construction of train agent and the design of operation adjustment. The control strategy based on rules for the operation adjustment is proposed. The simulation shows the Multi-agent technology is suitable to the subway operation adjustment, it has the ability to extend dynamically.