The nucleotide imbalance and the nucleotide pair imbalance signals of hominidae mitochondrial nucleotide genomic signals (mtNuGSs) have specific features for the areas encoding the 13 mt proteins, the 22 mt transfer RNAs (mt tRNA), the two mt ribosomal RNAs (mt rRNA), or for the segment(s) corresponding to the D-loop. The nucleotide genomic signal analysis of mt DNA allows identifying such features, and when applied to samples from remains of homo sapiens and other hominidae it can leads to a precise identification of their sources.