In order to improve the measuring speed and accuracy of the surgical instruments inspection, a computer-aided measuring and analysis method was highly needed. The purpose of this study was provide surgical instruments manufactures with a surgical and microsurgical instruments inspection system, so as to ensure that surgical instruments came from assembly lines were manufactured correctly. Software and hardware components were designed and developed into a machine vision inspection system. Different surgical instruments such as scissors, forceps, clamps, microsurgical instruments were inspected; the system accuracy could be 0.001 mm. The result showed that the detection system had significant effects. The detection system could measure the vast amount of measurements of surgical instruments required for mass-produced devices.