A real-time strain monitoring system based on distributed Fiber Bragg Grating sensors is designed for the health monitoring of port machinery. This paper describes the system design and field test on port machinery in Qing Huangdao and the test results are discussed. Through the long-term monitoring on running state especially the structure strain of port machinery, the status of the port machinery including security and fatigue life could be acquired and estimated exactly, which could provide the foundation for the use and maintenance of port machinery and improve the security of the port machinery. Long-term test results showed good linearity and repeatability.