Many forms of spinal fusion involve the placement of long screws through the pedicles into the vertebral body. During the procedure, there is substantial risk of damage to vital neural and vascular structures due to the limited visibility of anatomic landmarks and high anatomic variability. As an alternative to current guidance systems, we have investigated the feasibility of performing ultrasound imaging through cancellous bone for the purpose of pedicle screw guidance. Quantitative ultrasonic characterization and A-mode imaging of seven defatted vertebral cancellous bone specimens was performed along the craniocaudal axis in water with unfocused, 1-MHz and 3.5- MHz broadband transducers. The center frequency attenuation increased considerably from 10.5 plusmn 4.6 dB/cm at 1 MHz to 24.1 plusmn 7.2 dB/cm at 3.5 MHz, while the speed of sound exhibited moderate positive dispersion, increasing from 1489 plusmn 4.7 m/s at 1 MHz to 1494 plusmn 4.2 m/s at 3.5 MHz. Despite the high attenuation and large specimen thickness (1.0-1.9 cm), A-mode imaging through cancellous bone to detect an aluminum reflector was possible in 83.2% and 70.1% of the cases at 1 MHz and 3.5 MHz, respectively. Specimen boundaries were identifiable with clinically sufficient average accuracy of 1.1 mm and 0.9 mm in the 1 MHz and 3.5 MHz A-mode images, respectively.