A new mechanical gas-tight sampler has been designed to collect hydrothermal fluids at the seafloor. A key feature of the sampler is the novel sample valve which is pressure balanced under deep sea, and actuated by the ram on a submersible's manipulator. The sampler is designed to be deployed at the seafloor 3000 m underwater and can be used to sample hydrothermal fluid with temperature up to 400degC. Compressed gas is used to compensate for pressure reduction of sample fluids. Simulation of the flow system was conducted to estimate the fill rate. The sampler has been tested successfully in the first Sino-American Cooperative Deep Submergence Project from August 13 to September 3, 2005.