In this study, we propose a Genetic Extended-fiber Network (GEN) separately stretched over microbridges for DNA fiber applications. The microbridges are rapidly and easily fabricated by directly spin-coating a thick negative photoresist on a metal mask patterned glass substrate and exposing it twice from the backside of the substrate for two different exposure angles. The GEN constructed over microbridge array by centrifugal stretching can achieve high throughput stretching-and-positioning of DNA fibers and individually accessible platform for DNA fibers, on which spatial relationships of DNA fibers can be quantitatively analyzed by accurate compartmentalization.