In this paper simultaneous detection of fluorescence and turbidity using on-chip multi wavelength detection sensor was reported. The sensor was fabricated in 5 mum, single-poly, double-metal, p-well CMOS technology. At first, the capability of fluorescence and turbidity were confirmed. In the experiment of fluorescence detection, concentrations down to 37.5 nM have reliably been measured. In the experiment of turbidity detection, the comparable results with Si photodiode were detected. Finally, the sensor was applied for real time monitoring of a loop-mediated isothermal amplification method (LAMP). The fluorescence and turbidity change were successfully observed.