MIMO-OFDM system is a promising way to achieve high data-rate wireless transmission. In this paper, loosely synchronous (LS) codes assisted multipath channel interference canceller (MCIC) is proposed for the channel estimation of MIMO-OFDM systems. Since LS codes have perfect autocorrelation and cross-correlation functions within certain vicinity of the zero shifts, LS codes assisted MCIC can substantially reduce multipath channel interference. Simulation results show that the MCIC estimator outperforms least square estimator and has slightly inferior performance as compared with linear minimum mean square error (LMMSE) estimator. Moreover the MCIC estimator has the advantage of not requiring any information about the channel statistics like auto-correlation of channels.