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Badano zdolność indukowania uszkodzeń DNA plazmidu pUC19 przez powszechnie
stosowany insektycyd fosforoorganiczny metyloparation i jego główny metabolit metyloparaokson
po 72 h inkubacji z tymi związkami. Stosowano metodę elektroforezy w żelu agarozowym
i analizowano zmianę intensywności pasm odpowiadających dwóm formom konformacyjnym
plazmidu - kolistej superskręconej (CCC) i kolistej otwartej...
The activities of intercellular antioxidative enzymes: Superoxide dismutase, catalase and
glutathione peroxidase were estimated in control and trisomic human fibroblasts in culture.
It was found that in trisomic cells the activity of SOD is approximately 50% higher than in
control cells. The activity of glutathione peroxidase is increased by 30% in trisomic fibroblasts,
whereas catalase activity...
The destructive role of reactive oxygen species (ROS) in cell and tissue damage is well
documented. However, so far a very little is known about their role as a regulating factor
in the cellular metabolism. At present, we cannot judge out whether the superoxide radicals
are beneficial or harmful to the organisms. Their positive or negative influence each time is
determined by a numerous factors...
In this work the effect of Paraquat (PQ) and Graraoxone (GX) on isolated human and
carp hemoglobins was studied. Degradation products of PQ were isolated from GX solution
by thin layer chromatography on silica gel platelets. Purity of the GX fractions obtained was
verified on the basis of silica gel rechromatography. The fractions were identified by IR, UV
and visible spectra. Binding of PQ degradation...
The phenoxyherbiddes are commonly used in Poland. In great numbers the herbicides
can reach the environment and be accumulated in living organisms. The reactions of the
degradations of those herbicides may lead to create many of their derivatives. Some of them
may demonstrate greater degree of their toxical action in compare with original substrate.
Among many of the toxical effects we should...
Enzymy restrykcyjne ze względu na istotę swojego działania mogą być źródłem informacji
na temat miejsca lub specyficzności sekwencyjnej wiązania substancji do DNA. Z drugiej
strony, działanie enzymów może być zaburzone przez związki mające zdolność do metylacji
zasad DNA. Cecha ta może być wykorzystywana do pierwotnej selekcji związków potencjalnie
genotoksycznych. W pracy badano działanie...
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