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The control of expression of the α-toxin gene (cpaorplc) ofClostridium perfringenshas been studied in three strains shown to have high (NCTC8237), intermediate (strain 13) and low (NCTC8533) phospholipase C activity in the culture supernatant. The phospholipase C activity was shown to be related tocpamRNA levels. Primer extension studies were performed to locate thecpapromoter regions in strains NCTC8237...
Cellulolytic and xylanolytic activities among genera of anaerobic fungi when grown on glucose, xylan and the cellulosic substrates, filter paper and Avicel were compared. All the fungi had basal extracellular fibrolytic activities that could be enhanced by growth on xylan or the cellulosic substrates. However,Piromyces communisstrain 22 andNeocallimastix patriciarumstrain 27 had substantially greater...
Using a DNA probe originally designed for detection of sigma factor genes inC. acetobutylicum, a 4722-bp fragment of clostridial chromosomal DNA was cloned and sequenced. Three complete open reading frames (ORFs) and a fourth truncated ORF at the 5′ end of the fragment could be identified. The deduced amino acid sequence of the truncated ORF showed significant similarity to thekdpCgene ofEscherichia...
From samples collected in crude oil storage tanks and oil production waters near the coastal town of Vung Tau in Southern Vietnam, several strains of sulfate-reducing bacteria were isolated and maintained in pure culture. Strain G3 100 consisted of vibrio-shaped, motile, non-sporulating, Gram-negative, desulfoviridin-containing cells. It grew optimally at 5% NaCl. It utilized a range of organic acids...
The clostridia are a diverse group of obligately anaerobic bacteria with potential for the fermentative production of fuels, solvents and other chemicals. Several species exhibit a broad substrate range, but there have been few studies of the mechanisms involved in regulation of uptake and metabolism of fermentable carbohydrates.Clostridium beijerinckii(formerlyClostridium acetobutylicum) NCIMB 8052...
Experiments were conducted to evaluate the effects of different competitive exclusion (CE) cultures on the concentration of cecal propionic acid in 3-day-old broiler chicks, and the correlation between cecal propionic acid concentration and protection againstSalmonellacolonization. CE cultures that significantly (P<0.05) increased cecal propionic acid in 3-day-old chicks decreased (P<0.05) cecalSalmonellacolonization...
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